منابع مشابه
[Transcription of the lactose operon in Escherichia coli mutants].
Hi protein, a heat-stable low-molecularweight DNA-binding factor previously described by CukierKahn et al. [Proc. Nat. Acad. Sci. USA (1972) 69, 3643-;36471 markedly stimulates in vitro synthesis of lac-specific RNA directed by bacteriophage Xh80 dlac or 480 dlac DNA templates in the presence of purified E. coli RNA polymerase holoenzyme. The extent of stimulation obtained by addition of H1 alo...
متن کاملCyclic adenosine monophosphate-independent mutants of the lactose operon of Escherichia coli.
In Escherichia coli the transcription of the lactose operon, like other catabolite-sensitive operons, requires catabolite gene activator protein and 3',5'-cyclic adenosine monophosphate in addition to ribonucleic acid polymerase. We isolated and analyzed lac(+) revertants from a crp(-) strain of E. coli. We found that revertants with a higher level of expression only for the lac operon lie in t...
متن کاملTranscriptional regulation and evolution of lactose genes in the galactose-lactose operon of Lactococcus lactis NCDO2054.
The genetics of lactose utilization within the slow-lactose-fermenting Lactococcus lactis strain NCDO2054 was studied with respect to the organization, expression, and evolution of the lac genes. Initially the beta-galactosidase gene (lacZ) was cloned by complementation of an Escherichia coli mutant on a 7-kb HpaI fragment. Nucleotide sequence analysis of the complete fragment revealed part of ...
متن کاملBistability of the lac operon during growth of Escherichia coli on lactose and lactose+glucose.
The lac operon of Escherichia coli can exhibit bistability. Early studies showed that bistability occurs during growth on TMG/succinate and lactose+glucose, but not during growth on lactose. More recently, studies with lacGFP-transfected cells show bistability during growth on TMG/succinate, but not during growth on lactose and lactose+glucose. In the literature, these results are invariably at...
متن کاملKinetic approaches to lactose operon induction and bimodality.
The quasi-equilibrium approximation is acceptable when molecular interactions are fast enough compared to circuit dynamics, but is no longer allowed when cellular activities are governed by rare events. A typical example is the lactose operon (lac), one of the most famous paradigms of transcription regulation, for which several theories still coexist to describe its behaviors. The lac system is...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2002
ISSN: 1470-8175,1539-3429
DOI: 10.1002/bmb.2002.494030060092